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Nanotemper Technologies GmbH


NanoTemper Technologies GmbH

Dr. Stefan Duhr
Dr. Philipp Baaske

Amalienstraße 54
80799 München
Tel.: 089-2180 2833




Based on the proprietary technology “Microscale Thermophoresis (MST)”, the company is developing affordable analytical instruments for the characterization of biomolecule interactions and for the study of biomolecule activity in vitro. The instruments are suited for basic research and pharmaceutical applications.
As a spin-off from the Biophysics Department of the Ludwig-Maximilians-University Munich, NanoTemper consists of an interdisciplinary team of physicists, biologists and biochemists with a strong background in Microscale Thermophoresis and in biomolecule analytics.


Monolith NT.115 is NanoTemper´s MST Instrument for basic research and pharmaceutical applications.




Microscale Thermophoresis is a powerful new technology, and easy to handle. It detects changes in the hydration shell of molecules and is ideal for measuring enzyme activity and biomolecule interactions under close-to-native conditions: immobilization-free and in complex biological liquids like blood serum or cell lysate

NanoTemper´s unique technology is ideal for basic research applications requiring flexibility in the experimental scale, as well as for pharmaceutical research applications, including small molecule profiling, which are difficult to access with established technologies as they require a high sensitivity.


By using IR-lasers, precise microscale temperature gradients can be produced within capillaries. Molecules show a directed motion along these temperature gradients. The thermophoretic motion is determined by the hydration shell of the molecules. Any change of the hydration shell of biomolecules due to changes in their primary, secondary, tertiary and/or quaternary structure affects the thermophoretic movement and can be used to determine binding affinities, binding kinetics and enzymatic activities with high accuracy and sensitivity. The interaction of biomolecules and microparticles can be measured under close-to-native-conditions, immobilization-free and in a liquid of choice, including blood serum, cell lysate or reaction buffer.